elastase (Worthington Biochemical)
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Elastase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 95/100, based on 178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/elastase/Elastase%2C+Lyophilized/pm42882460-73-45-48
Average 95 stars, based on 178 article reviews
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Gentle:Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves. Article Snippet: .. https://doi.org/10.3390/cells15191762 Tissues were placed in warm Tyrode solution containing (in mmol/L) 140 NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized Single Cell:Article Title: Trp53 heterozygosity defines novel states impacting tumor plasticity and progression in Kras-initiated non-small cell lung cancer Article Snippet: Tumor-bearing whole lungs were harvested into Gibco Leibovitz’s L-15 Medium (Gibco, cat. no. 21083-027) and finely minced using forceps under the dissection microscope. .. To obtain single-cell suspensions, minced tissue was subjected to both enzymatic digestion in L-15 medium supplemented with 2mg/mL Collagenase Type I (Worthington, cat. no. LS004197), 2mg/mL Article Title: Tectorigenin reprograms macrophages through targeting OXCT1-mediated ketone metabolism to suppress IFN-I signaling and ameliorate myocardial ischemia-reperfusion injury Article Snippet: Stain with the following antibodies: CD68 polyclonal antibody (1:500, Cat. No.: 28058-AP-1; Proteintech Group, Inc., Wuhan, China); BHLHE41 polyclonal antibody (1:50, PA5-25651; Thermo Fisher Scientific, Inc.); OXCT1 polyclonal antibody (1:400, Cat. No.: PA5-146043; Thermo Fisher Scientific, Inc.); Flag antibody (1:800, Cat. No.: #14793; Cell Signaling Technology, Inc.); and lysosomal associated membrane protein 1 (LAMP1) polyclonal antibody (1:400, Cat. No.: PTM-5775; PTM Biolab, Inc., Hangzhou, China). .. After cardiac perfusion of the mice, the left ventricle was excised, minced, and digested in PBS with a cocktail of 1.5 mg/mL type II collagenase (Cat. No.: LS004176; Worthington Biochemical Corporation, Lakewood, NJ, USA), 0.25 mg/mL Tissue:Article Title: Trp53 heterozygosity defines novel states impacting tumor plasticity and progression in Kras-initiated non-small cell lung cancer Article Snippet: Tumor-bearing whole lungs were harvested into Gibco Leibovitz’s L-15 Medium (Gibco, cat. no. 21083-027) and finely minced using forceps under the dissection microscope. .. To obtain single-cell suspensions, minced tissue was subjected to both enzymatic digestion in L-15 medium supplemented with 2mg/mL Collagenase Type I (Worthington, cat. no. LS004197), 2mg/mL Article Title: Tectorigenin reprograms macrophages through targeting OXCT1-mediated ketone metabolism to suppress IFN-I signaling and ameliorate myocardial ischemia-reperfusion injury Article Snippet: Stain with the following antibodies: CD68 polyclonal antibody (1:500, Cat. No.: 28058-AP-1; Proteintech Group, Inc., Wuhan, China); BHLHE41 polyclonal antibody (1:50, PA5-25651; Thermo Fisher Scientific, Inc.); OXCT1 polyclonal antibody (1:400, Cat. No.: PA5-146043; Thermo Fisher Scientific, Inc.); Flag antibody (1:800, Cat. No.: #14793; Cell Signaling Technology, Inc.); and lysosomal associated membrane protein 1 (LAMP1) polyclonal antibody (1:400, Cat. No.: PTM-5775; PTM Biolab, Inc., Hangzhou, China). .. After cardiac perfusion of the mice, the left ventricle was excised, minced, and digested in PBS with a cocktail of 1.5 mg/mL type II collagenase (Cat. No.: LS004176; Worthington Biochemical Corporation, Lakewood, NJ, USA), 0.25 mg/mL Suspension:Article Title: Tectorigenin reprograms macrophages through targeting OXCT1-mediated ketone metabolism to suppress IFN-I signaling and ameliorate myocardial ischemia-reperfusion injury Article Snippet: Stain with the following antibodies: CD68 polyclonal antibody (1:500, Cat. No.: 28058-AP-1; Proteintech Group, Inc., Wuhan, China); BHLHE41 polyclonal antibody (1:50, PA5-25651; Thermo Fisher Scientific, Inc.); OXCT1 polyclonal antibody (1:400, Cat. No.: PA5-146043; Thermo Fisher Scientific, Inc.); Flag antibody (1:800, Cat. No.: #14793; Cell Signaling Technology, Inc.); and lysosomal associated membrane protein 1 (LAMP1) polyclonal antibody (1:400, Cat. No.: PTM-5775; PTM Biolab, Inc., Hangzhou, China). .. After cardiac perfusion of the mice, the left ventricle was excised, minced, and digested in PBS with a cocktail of 1.5 mg/mL type II collagenase (Cat. No.: LS004176; Worthington Biochemical Corporation, Lakewood, NJ, USA), 0.25 mg/mL |
